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GnRHa和孕三烯酮对异位子宫内膜细胞生长及凋亡的影响 被引量：5

Effects of GnRHa and Gestrinone on growth and apoptosis in ectopic endometrial stroma cells
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摘要 目的探讨GnRHa及孕三烯酮对体外培养的异位子宫内膜间质(ES)细胞生长和凋亡的影响,及两种药物作用后基质金属蛋白酶-3(MMP-3)在ES细胞表达的变化。方法以不同浓度的GnRHa和孕三烯酮对体外培养的ES细胞分别进行处理,倒置相差显微镜下观察细胞的生长状态,免疫组化法鉴定间质细胞。采用四甲基偶氮唑蓝(MTT)比色法检测药物作用0~60 h的细胞抑制情况,并绘制细胞生长曲线;采用流式细胞仪检测GnRHa和孕三烯酮作用48h后异位内膜间质细胞周期和凋亡率的变化。采用免疫组化法检测不同浓度GnRHa和孕三烯酮作用48h后MMP-3在异位内膜表达的变化。结果GnRHa和孕三烯酮可抑制细胞的生长增殖,并呈时间和剂量依赖方式。药物作用48 h后,各组细胞均发生细胞周期阻滞,G1期细胞增加,S期细胞减少,各组凋亡率明显升高(P<0.05);免疫组织化学表明,两种药物均可降低异位子宫内膜MMP-3的表达;且GnRHa诱导异位子宫内膜细胞的凋亡、降低其MMP-3表达的作用强于孕三烯酮(P<0.05)。结论GnRHa和孕三烯酮均可明显抑制体外培养的ES细胞的生长增殖,诱导其凋亡,同时降低MMP-3的表达,且GnRHa上述作用... Objective To investigate the effects of GnRHa and Gestrinone on growth,proliferation and apoptosis in isolated ectopic endometrial stroma cells in vitro,as well as expression of MMP-3 induced by GnRHa and Gestrinone.Methods Axenic ectopic endometriotic tissues were isolated and cultured in vitro.Then the cultured endometrial stroma cells were observed by an inverted microscope and identified by vimentin immunohistochemical staining.They were cultured and exposed to GnRHa and Gestrinone at different doses.Th...
作者 王春凤 姜卫国
机构地区 山东大学医学院山东省千佛山医院妇产科
出处 《山东大学学报（医学版）》 CAS 北大核心 2009年第2期70-73,共4页 Journal of Shandong University:Health Sciences
关键词 子宫内膜异位症 GNRHA 孕三烯酮 细胞周期 细胞凋亡 Endometriosis GnRHa Gestrinone Cell cycle Cell apoptosis
分类号 R711.71 [医药卫生—妇产科学]
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  • 1Sugawara J,Yoshida H,Fukaya T,et al.Increased secretion of hepatocyte growth factor by e utopic endometrial stromal cells in women with endometriosis[J].Fertil Steril, 1997,68:468-472
  • 2Oventon CE,Barolw DH,Fernandez-Shaw S,e t al.In vitro culture of endometrial stormal and gland cells as a model for endo metriosis:The effect of peritoneal fluid on proliferation[J].Fertil Steril,199 7,67:51-56
  • 3Sharpe KL,Zimmer RL,Khan RS,et al.Proliferativ e and morphogenic changes induced by the coculture of rat uterine and peritoneal cells:A cell culture model for endometriosis[J]. Fertil Steril,1992,58:1220- 1229
  • 4Arici A,Senturk LM,Seli E,et al.Regulation of monocyt e chemotactic protein-1 expression in human endometrial stromal cells by estrog en and progesterone[J].Biol Reprod,1999,61:85-90
  • 5Ryan I P,Schriock ED,Taylor RN.Isolation,characterization,and comparison of human endom etrial and endometriosis cells in vitro[J].J Clin Endocriol Metab,1994,78:642 -649
  • 6Sharpe KL,Zimmer RL,Griffin WT,et al.Polypeptides sy nthesized and released by human endometriosis tissue differ from those of the ut erine endometrium in culture[J].Fertil Steril,1993,60:839-851
  • 7Sharpe-Timms K.Basic researches in endometriosis[J].Obstet Gynecol Cli n of North America,1997,242:296-290
  • 8Matthws GJ,Redfern CP F,Hirst BH,et al.Characterization of human purified and stromal cells from endom etrium and endometriosis in tissue culture[J].Fertil Steril,1992,57:990-997

共引文献74

同被引文献35

  • 1喇端端.卵巢子宫内膜异位囊肿的腹腔镜手术治疗[J].中国微创外科杂志,2007,7(3):199-200. 被引量：13
  • 2Vercellini P, Crosignani PG, Fadini R et al. A gonadotrophin - releasing hormone agonist compared with expectant managcment after conservative surgery for symptomatic endometriosis [ J ] . Br J Obstet Gynaecol, 1999, 106 (7): 672.
  • 3The Practice Committee of the American Society for Reproductive Medicine. Endometriosis and infertility [J] . Fertil Steril, 2004, 82: 14412.
  • 4Agarwol SK. Comparative effects of GnRH agonist therapy: review of clinical studies and their implications[J].J Reprod Med, 1998, 43 (3) : 293.
  • 5乐杰.妇产科学[M].第6版.北京:人民卫生出版社,2003:319-329.
  • 6Li CZ, Liu B, Wen ZQ, et al. The impact of electrocoagula-tion on ovarian reserve after laparoscopic excision of ovariancysts: a prospective clinical study of 191 patients ( J) . FertilSteril,2009, 92 (4) : 1428.
  • 7M.Bilotas,R.I.Baranao,R.Buquet, et al.Effect of GnRH analogues on apoptosisndexpression of Bcl-2, Bax, Fas and FasL Proteins in endom etrial epithelial cellcultures from patients with endometriosis and controls[].Human Reproduction.2007
  • 8Khan KN,Kitajima M,Hiraki Ket al.Cell proliferation effect of GnRH agonist on pathological lesions of women with endometriosis, adenomyosis and uterine myoma[].Human Reproduction.2010
  • 9Kim NY,Ryoo U,Lee DY, et al.The efficacy and tolerability of short-term low-dose estrogen-only add-back therapy during post-operative GnRH agonist treatment for endometriosis.[].European Journal of Obstetrics Gynecology and Reproductive Biology.2011
  • 10Post S,Peeters W,Busser E,et al.Balance between angiopoietin-1and angiopoietin-2is in favor of angiopoietin-2in atherosclerot-ic plaques with high microvessel density[].Journal of Vascular Research.2008

引证文献5

二级引证文献38

山东大学学报（医学版）
2009年 第2期

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