摘要
目的探讨GnRHa及孕三烯酮对体外培养的异位子宫内膜间质(ES)细胞生长和凋亡的影响,及两种药物作用后基质金属蛋白酶-3(MMP-3)在ES细胞表达的变化。方法以不同浓度的GnRHa和孕三烯酮对体外培养的ES细胞分别进行处理,倒置相差显微镜下观察细胞的生长状态,免疫组化法鉴定间质细胞。采用四甲基偶氮唑蓝(MTT)比色法检测药物作用0~60 h的细胞抑制情况,并绘制细胞生长曲线;采用流式细胞仪检测GnRHa和孕三烯酮作用48h后异位内膜间质细胞周期和凋亡率的变化。采用免疫组化法检测不同浓度GnRHa和孕三烯酮作用48h后MMP-3在异位内膜表达的变化。结果GnRHa和孕三烯酮可抑制细胞的生长增殖,并呈时间和剂量依赖方式。药物作用48 h后,各组细胞均发生细胞周期阻滞,G1期细胞增加,S期细胞减少,各组凋亡率明显升高(P<0.05);免疫组织化学表明,两种药物均可降低异位子宫内膜MMP-3的表达;且GnRHa诱导异位子宫内膜细胞的凋亡、降低其MMP-3表达的作用强于孕三烯酮(P<0.05)。结论GnRHa和孕三烯酮均可明显抑制体外培养的ES细胞的生长增殖,诱导其凋亡,同时降低MMP-3的表达,且GnRHa上述作用...
Objective To investigate the effects of GnRHa and Gestrinone on growth,proliferation and apoptosis in isolated ectopic endometrial stroma cells in vitro,as well as expression of MMP-3 induced by GnRHa and Gestrinone.Methods Axenic ectopic endometriotic tissues were isolated and cultured in vitro.Then the cultured endometrial stroma cells were observed by an inverted microscope and identified by vimentin immunohistochemical staining.They were cultured and exposed to GnRHa and Gestrinone at different doses.Th...
出处
《山东大学学报(医学版)》
CAS
北大核心
2009年第2期70-73,共4页
Journal of Shandong University:Health Sciences