摘要
目的探讨化淤理肺方对肺纤维化的干预作用和对MMP-2/TIMP-1表达的影响。方法肺纤维化Wistar雄性大鼠84只,随机分成4组:中药组、激素组、模型组和对照组。所有大鼠第7、14、28天各处死7只。经HE染色评价肺组织肺泡炎和肺纤维化程度,免疫组化检测法检测MMP-2与TIMP-1的表达。结果①肺泡炎、肺纤维化程度评价:模型组肺泡炎和肺纤维化程度较重,且胶原纤维沉积较多;中药组可见炎性细胞较少,肺泡结构基本正常,肺泡炎与肺纤维化较轻;激素组肺泡炎及纤维化与中药组相似。②MMP-2蛋白的表达:中药组第7、142、8天较模型组表达明显减轻,于第14天达到高峰,此后降低,表达水平与激素组相似。③TIMP-1蛋白表达:中药组在这3个时段的表达均较对照组增高,但均较模型组低,且呈逐渐升高趋势。结论化淤理肺方能减轻博来霉素诱导的大鼠肺纤维化,可能是通过调节MMP-2、TIMP-1途径起作用。
Objective To evaluate the effects and mechanism of Huayulifei on blemycin-induced pulmonary interstitial fibrosis in rats.Methods Rats were randomly divided into the control group,the model group,the Huayulifei group and the hydrocortisone group.Rat pulmonary fibrosis was induced by blemycin(5 mg/kg) in the experimental groups and 0.9% saline in the control group.Rats were killed on the 7th,14th and 28th days.Expressions of MMP-2 and TIMP-1 proteins were determined by an immunohistochemical technique.Result...
出处
《山东大学学报（医学版）》
CAS
北大核心
2009年第2期14-17,共4页
Journal of Shandong University:Health Sciences
基金
山东省科技攻关课题(2006GG2302030)。
