结核分枝杆菌Ag85B蛋白的重组及表达

Cloning and Expression of the Ag85B Protein from Mycobacterium Tuberculosis

目的对结核分枝杆菌Ag85B蛋白进行重组表达,初步评价其免疫反应性。方法用PCR方法得到Ag85B蛋白的编码基因,构建重组质粒,转化到大肠杆菌BL21中。经IPTG诱导表达,得到高效稳定的表达株,并用蛋白免疫印迹方法检测免疫反应性。结果获得了高效稳定的重组Ag85B的表达株,并且重组Ag85B有较好的免疫反应性。结论重组Ag85B有较好的免疫反应性,为结核病快速诊断研究及新疫苗研究提供了基础。

Objective To clone and express Ag85B,and to evaluate the feasibility of the recombinant protein as a diagnosis antigen. Methods The piece of genes coding Ag85B was obtained by PCR method.Recombinant plasmid was constructed, then, transformed into E.coli BL21 strain, and induced by IPTG.The expression product was analyzed with Western-blot method. Results The recombinant protein expressed in host bacteria was effective .By means of western-blot assay it was showed that immunological response was good. Conclu...