天麻多糖分离、结构分析与自由基清除作用研究

Study on Extraction Technology,Structure and Free Radical Scavenging Activity of Polysaccharides from Gastrodia elata B1

目的:研究活性天麻多糖的提取、结构分析与自由基清除作用。方法:以水提醇沉法制备天麻粗多糖,CTAB季铵盐络合法、凝胶过滤柱层析进一步纯化;通过IR、NMR等分析天麻多糖结构;利用Feton体系和邻苯三酚自氧化反应,采用比色法测定天麻多糖对.OH及O-2.的清除作用。结果:通过正交试验获得天麻多糖提取工艺为:料水比1:40,浸提温度80℃,提取次数2次,75%乙醇沉淀;红外光谱和1H-NMR分析天麻多糖为α-吡喃己糖,单糖分析结果显示它为单一葡萄糖残基组成,13C-NMR分析表明天麻多糖为带1→6键接支链的α-(1→4)-D-葡聚糖;在相同的实验体系浓度下,天麻粗多糖及纯化多糖对.OH的IC50分别为1.18、1.62mg/ml;对O-2.的IC50分别为0.81、1.03mg/ml。结论:天麻多糖为1→6键接支链的α-(1→4)-D-葡聚糖,对O-2.具有一定清除能力。

Polysaccharides were extracted from Gastrodia elata B1 with water and precipitated by ethanol,purified through cetyltrimethylammonium bromide(CTAB) complex method and Sepharose 6-fast flow chromatography.The structural charac-terization of Gastrodia elata B1 polysaccharides(GPS) was conducted by means of IR and NMR techniques.In simulated chemical reaction system,the scavenging effects of GPS on.OH and O-2.were investigated via Fenton reaction and pyrogallol autoxidation method repectively.Results showed th...