摘要
本研究以木瓜等水果果肉为实验材料,对改进的CTAB提取方法进行优化,探讨适宜的果肉冷冻干燥粉的量和聚乙烯吡咯烷酮(PVP)的添加量。最终确定加入0.10g果肉冷冻干燥粉较为合适,提取缓冲液中PVP添加1.0%时效果较好。此方法有效的去除了水果果肉中多酚和多糖类物质的影响,提取出的DNA浓度较高,A260/A280值介于1.80～2.00之间。通过比较,改进的CTAB法优于SDS法和DNA提取试剂盒法。以所提取出的木瓜、哈密瓜、香蕉、梨果肉总DNA为模板,对内源rbcL基因进行PCR扩增,均得到了433bp大小的目的条带。
This study optimized the method of modified CTAB from fruit fleshes such as papaya,ect.The results showed that the optimum amount of lyophilized fruit powder is 0.10 g and the best proportion of polyvinyl pyrrolidone(PVP) added into extraction buffer is 1.0%.Furthermore,by this method,the effects of polyphenolics and polysaccharides in fleshes are effectively removed,the concentration of isolated genomic DNAs is high,and the value of A260/A280 is between 1.80 and 2.00.Compared to SDS method and DNA extracti...
出处
《食品科学》
CAS
CSCD
北大核心
2009年第3期231-234,共4页
Food Science
关键词
转基因
水果
DNA提取
genetically modified organism（GMO）
fruits
DNA extraction
