奶粉中阪崎肠杆菌的污染及快速检测方法的研究

Study on Enterobacter sakazakii Contamination in Infant Formula Milk Powder and Its Rapid Detection Method

目的:了解福建省市售婴幼儿配方奶粉中阪崎肠杆菌的污染状况、污染途径并寻找快速准确的检测方法。方法:应用分离鉴定、PCR和荧光PCR等方法检测,分离鉴定采用阪崎肠杆菌显色培养基和全自动微生物生化仪。结果:阪崎肠杆菌在192份市售婴幼儿配方奶粉中的检出率为1.56%,在60份原料奶粉中的检出率为13.33%,30份生产车间环境样本均未检出。结论:福建省市售婴幼儿配方奶粉中存在阪崎肠杆菌的安全隐患,某工厂奶粉中阪崎肠杆菌的污染主要来自原料奶粉。荧光PCR和显色培养基可用于阪崎肠杆菌的快速筛选和分离。

Objective:To realize the status of Enterobacter sakazakii contamination in infant formula milk powder,and to study relevant contamination routes and rapid detection methods.Methods:Enterobacter sakazakii colored medium and VITEK automatic microbial biochemical analyzer were used in isolation and identification,PCR and fluorescence PCR were used for detection.Results:The detection rate of Enterobacter sakazakii is 1.56% in 192 infant formula milk powder product samples,13.33% in 60 material samples and 0% in...