乳源性促矿物元素吸收肽超滤技术的优化

Optimization of Ultra-filtration Technology of Milk-derived Mental-binding Peptides

采用超滤技术,对通过胰蛋白酶酶解制备的粗肽进行初步分离纯化,从而获得较高纯度的促矿物元素吸收肽。对超滤工艺中几个主要技术参数(超滤压力、料液初始稀释倍数、料液pH值、料液温度)进行了研究,以膜通量、目标肽回收率和P/N(摩尔比)作为其结果的评价指标。结果表明:利用截留分子量为6000D的中空纤维超滤膜,在超滤压力为0.125MPa、料液初始稀释倍数为4、料液pH5.5、料液温度45℃的条件下,以45min作为一个超滤周期,能得到纯度最高和活性最大的促矿物元素吸收肽。该条件下获得的目标肽可以将NaH2PO4和CaCl2在体外产生沉淀的时间推迟15min,此时目标肽回收率为84.1%。

This study aimed to simply purify mental-binding peptides which were derived from hydrolyzed whey protein by ultra-filtration technology.The main technical parameters were studied,including ultra-filtration pressure,dilution multiple of liquid material,its pH value and temperature,taking membrane flux,peptide recovery rate and initial molar ratio of P to N as evaluation indexes.The results indicated that high-purity and high-activity mental-binding peptides can be obtained a ultra-filtration circle of 45 mi...