原子荧光光谱法测定大豆中不同形态的硒

Determination of Organic and Inorganic Selenium in Soybean by Atomic Fluorescence Spectrometry

本实验建立了一套快速、准确测定大豆中有机态硒和无机态硒的方法。研究了仪器的工作条件、载流、硼氢化钾浓度对硒荧光信号值的影响,消除了共存离子对硒测定时的干扰,利用本法成功地对大豆中的硒进行形态分析,方法的检出限为3.6ng/L,回收率为86.7%～105.0%,精密度为1.95%～4.52%。

This article studied effects of conditions of instrument,current-carrying and potassium borohydride concentration on fluorescence single value of selenium,and established a quick and accurate method to determine organic and inorganic selenium in soybean.This method successfully eliminated the disturb of coexisting ions on the detection of selenium,and the results showed that the detection limit is 3.6 ng/L,the recovery is 86.7% to 105.0%,and the relative standard deviation is 1.95% to 4.52%.