ZD1839对小鼠宫颈癌细胞株U14移植瘤抑制作用的研究

Study of growth-inhibition induced by ZD1839 on murine cervical carcinoma U14

[目的]观察ZD1839对小鼠宫颈癌U14的抗癌作用及对U14细胞凋亡的诱导作用,探讨ZD1839治疗宫颈癌的临床应用价值。[方法]建立615近交系小鼠宫颈癌U14移植瘤动物模型,随机分为4组(每组8只):A组为空白对照组,给予0.5 mL5%葡萄糖溶液灌胃,B、C、D组分别给予50、100、150 mg/(kg.d)剂量的ZD1839进行灌胃治疗。检测各组小鼠的瘤体直径、重量和抑瘤率;采用流式细胞术和TUNEL法检测瘤体的细胞凋亡率。[结果]①A、B、C、D组小鼠的瘤体直径逐渐变小,肿瘤重量逐渐减轻(A与B组比较P>0.05,C、D组与A、B组比较P<0.05);A、B、C、D组肿瘤抑制率分别为0、3.95%、24.86%、25.99%。②A、B、C、D组肿瘤细胞凋亡率:采用流式细胞术分别为(2.50±0.37)%、(3.52±0.32)%、(9.92±0.65)%、(15.69±0.70)%(C、D组与A、B组比较,C组与D组比较P<0.01);TUNEL法分别为(0.9±0.29)%、(2.87±0.73)%、(6.84±1.01)%、(11.77±1.24)%(B、C、D组与A组比较P<0.05,C、D组与B组比较,C组与D组比较P<0.01)。[结论]①ZD1839对小鼠宫颈癌移植瘤具有明显的抑瘤作用且具有剂量依赖性。②ZD1839通过引起宫颈癌细胞凋亡而抑制肿瘤生长,可通过TUNEL法检测肿瘤细胞凋亡率预测治疗效果。③ZD1839有可能成为宫颈癌患者的综合治疗方法之一。

[Objective]To observe the growth-inhibition and the apoptosis induced by ZD1839 on murine cervical carcinoma U14 and to investigate the value of ZD1839 in the treatment of cervical cancer.[Methods]Animals models of 615-strain mice with cervical cancer U14 were established.615-strain mice with U14 were randomly divided into four groups.A group was the blank control group,which was filled with 0.5 mL 5% glucose solution in stomach,B,C,D group was filled with separately 50,100,150 mg/(kg·d) dosage ZD1839 in st...