免疫小鼠乳腺乳铁蛋白基因表达变化研究

Relative expression of lactoferrin in the mammary gland of mice under immune state

研究小鼠不同生理阶段以及免疫条件下,乳腺内乳铁蛋白基因mRNA表达量的变化。选择120只健康昆明小白鼠,于分娩后4 d进行如下制剂处理:脂肪酶蛋白(Lipase)、空免疫刺激复合物(ISCOM)和Lipase。于分娩后8和12 d(即注射后4和8 d)用间接ELISA法测定乳中和血清中的特异性IgG含量,用Real Time PCR检测小鼠乳腺中乳铁蛋白基因的相对表达量。另外,正常生理状态乳腺内乳铁蛋白基因mRNA表达量的变化分别是在分娩后1、4、8和12 d进行检测。结果表明,正常状态下,小鼠分娩后1 d时,乳腺内乳铁蛋白基因mRNA表达量相对较高,而在分娩后4、8和12 d时较低;免疫条件下,乳腺内乳铁蛋白显著上升。免疫刺激可以引起小鼠乳腺乳铁蛋白基因表达上调。

Using mice as a model animal,we determined changes in the relative expression of lactorferrin gene in the mammary gland under different natural stages and the immune state.One hundred and twenty KunMing White mice were divided into four groups.They were treated differently at days 4 after parturition as follows: group A-injecting isotonic Na chloride,group B-injecting Lipase+isotonic Na chloride,group C-injecting ISCOM,and group D-injecting Lipase+ISCOM.Samples of blood,milk and mammary gland issues were co...