摘要
目的观察高糖刺激的大鼠肾小球系膜细胞结缔组织生长因子(CTGF)和膜型基质金属蛋白酶-1(MT1-MMP)的动态变化,探讨糖尿病肾病(DN)的发病机制。方法体外培养的大鼠HBZY-1肾小球系膜细胞分为正常糖对照组,高糖组和甘露醇高渗对照组,采用RT-PCR及Western印迹法分别检测CTGF和MT1-MMP的mRNA及蛋白的表达,用酶联免疫吸附法(ELISA)检测培养上清中IV型胶原的含量。结果与对照组相比,高糖组各时间点系膜细胞CT-GF表达明显上调,IV型胶原的分泌增加,且二者随时间持续增高;而MT1-MMP的表达则随时间呈明显下降趋势。结论高糖可诱导肾小球系膜细胞CTGF表达增加,同时抑制MT1-MMP的表达,二者可能参与DN中细胞外基质(ECM)代谢失衡过程。
Objective To observe the high-glucose induced dynamic changes of connective tissue growth factor (CTGF) and membrane-type 1 matrix metalloproteinase (MT1-MMP) in rat glomerular mesangial cells (GMCs).Methods Rat HBZY-1 GMCs were cultured and divided into 3 groups: normal concentration of D-glucose (NG) group, high concentration of D-glucose (HG) group and mannitol plus D-glucose (NG+M) group. The mRNA and protein expressions of CTGF and MT1-MMP were detected with semi-quantitative RT-PCR and Western blot. T...
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2009年第1期29-33,共5页
Chinese Journal of Histochemistry and Cytochemistry
基金
河北省自然科学基金(C2004000536)
关键词
糖尿病肾病
肾小球系膜细胞
结缔组织生长因子
膜型基质金属蛋白酶-1
Diabetic nephropathy
Glomerular mesangial cell
Connective tissue growth factor（CTGF）
Membrane-type 1 matrix metalloproteinase（MT1-MMP）
