针刺“内关”“中脘”“足三里”对背侧网状亚核神经元放电的影响

Effects of Electroacupuncture of "Neiguan"(PC 6),"Zhongwan"(CV 12) and "Zusanli"(ST 36) on Electrical Activities of Neurons in Subnucleus Reticularis Dorsalis in Rats

目的:观察腧穴"胃病方"即"内关""中脘""足三里"的针刺信号在背侧网状亚核传导与整合的途径。方法:选用健康成年雄性SD大鼠,10%乌拉坦腹腔注射麻醉,人工呼吸。立体定位仪固定头部,暴露菱形窝,玻璃微电极记录延脑背侧网状亚核(SRD)神经元细胞放电。首先记录30 s的神经元背景活动,然后施以手法(捻转角度120°、频率80次/min)刺激"内关"30 s,同时记录SRD神经元的反应;刺激结束,待细胞放电恢复后,再用相同手法分别刺激"中脘"与"足三里"穴。结果:刺激"内关"穴后,放电频率从(1.67±0.35)个/s增加到(17.55±1.40)个/s;刺激"中脘"穴后,放电频率从(2.35±0.43)个/s增加到(16.96±1.83)个/s;刺激"足三里"后,放电频率从(1.83±0.37)个/s增加到(17.39±1.58)个/s。结论:SRD可能为腧穴"胃病方"3穴在中枢传入信息聚合的部位之一。

Objective To compare the effects of electroacupuncture(EA) stimulation of "Gastric Problem Formula" ["Neiguan"(PC 6),"Zhongwan"(CV 12) and "Zusanli"(ST 36)] on electrical activities of neurons of medullary subnucleus reticularis dorsalis(SRD) of the rat.Methods Experiments were performed on SD rats anesthetized by 10% urethane(1.0-1.5 g/kg).Under artificial ventilation and immobilization(2% gallamine triethiodide,5 ml/kg),extracelluar discharges were recorded by glass micropipettes inserted into SRD of the ...