破骨细胞在强直性脊柱炎滑膜组织浸润及与软骨破坏的相关性

Osteoclast infiltration in synovium of ankylosing spondylitis and its relationship to cartilage damage

目的检测强直性脊柱炎(ankylosing spondylitis,AS)患者滑膜组织中破骨细胞浸润,探讨破骨细胞与软骨破坏相关性。方法采用酶组织化学技术检测滑膜组织内破骨细胞,Mankin评分检测软骨破坏情况,Leika Qwin高清晰图像分析系统测定阳性染色面积,相关分析分析破骨细胞表达与软骨破坏的相关性。结果破骨细胞在滑膜组织内阳性浸润,并主要分布在滑膜衬里层及滑膜与软骨交界处,阳性染色面积与对照组有显著差异,破骨细胞的浸润与软骨的破坏程度显著相关(r=0.654,P=0.001)。结论AS滑膜组织中有破骨细胞浸润,并与软骨的破坏程度具有相关性,在AS骨与软骨的破坏过程中发挥着重要的作用。

objective To detect the infiltration of osteoclast in synovium of ankylosing spondylitis(AS) and to explore the relationship of osteoclast to cartilage damage.Methods Using the enzymohistochemistry to detect the osteoclast phenotype in synovium of the AS;using Leika Qwin analytical system to determine positive staining areas.Degree of the cartilaginous damage in AS was observed with Mankin score;dependability between osteoclast and cartilage damage was evaluated with Spearman correlation analysis.Results Ta...