摘要
目的探讨宫颈癌中DAPK(Death-associated protein kinase)启动子甲基化与DNMT1 mRNA的表达及两者之间的关系。方法应用甲基化特异性PCR(methylation-specific PCR,MSP)技术检测52例宫颈癌、60例CIN(cervical intraepithelial neoplasia)和20例正常宫颈组织中DAPK启动子甲基化状况;应用原位杂交方法检测DNMT1 mRNA的表达。结果正常宫颈鳞状上皮不存在DAPK基因启动子CpG岛甲基化,而在CIN和宫颈癌中的甲基化率分别为18.3%(11/60)、65.4%(34/52),差异有统计学意义(χ2=39.639,P<0.001)。正常宫颈鳞状上皮、CIN和宫颈癌DNMT1 mRNA阳性率分别为10%、63.3%和78.8%,差异有统计学意义(χ2=29.057,P<0.001)。DAPK异常甲基化和DNMT1 mRNA表达成正相关,r=0.308,P<0.001。结论DAPK异常甲基化和DNMT1 mRNA过表达参与了宫颈癌的发生;DAPK基因启动子的甲基化过程可能是在DNMT1的催化作用下完成的。
Objective To explore the relationship between DAPK gene hypermethylation and DNMT1 gene expression in cervical cancer.Methods The promoter methylation of DAPK was investigated with Methylation-specific PCR in 52 cases of cervical cancer tissues,60 cervical intraepithelial neoplasia(CIN) cases,and 20 normal cervical tissues.DNMT1mRNA was detected by in situ hybridization.Results No methylation of DAPK gene was found in normal cervical tissues,while the methylation rate of DAPK promoter was 18.3%(11/60) in CI...
出处
《免疫学杂志》
CAS
CSCD
北大核心
2009年第1期80-82,87,共4页
Immunological Journal
