摘要
目的研究Foxp3及相关基因在重症肌无力(MG)胸腺组织中的表达,探讨其在MG发病中的作用及意义。方法采用免疫组织化学、Western blot以及RT-PCR技术,检测MG患者及正常对照胸腺组织中Foxp3、Smad3、Smad7、TGF-β、IFN-γ的表达差异。结果MG胸腺Foxp3蛋白及mRNA表达均显著低于正常,MGFAⅡ、Ⅲ型(全身型)患者胸腺Foxp3表达显著低于Ⅰ型(眼肌型);而Ⅲ型(中度全身型)则显著低于Ⅱ型(轻度全身型)(P<0.05);MG胸腺内TGF-βmRNA水平及其信使Smad3/Smad7比值显著降低,与Foxp3表达呈显著正相关(P<0.01,r=0.871)。结论Foxp3在MG发病中可能起着重要作用,TGF-β表达异常可能系MG胸腺Foxp3下调的原因之一。
Objective To explore the expression and clinic significance of gene Foxp3,TGF-β,IFN-γ at the myasthenic thymus.Methods Total of 50 myasthenic thymuses were examed for the expressions of Foxp3,TGF-β,IFN-γ,and Smad by immunohistochemistry staining(HIS),Western blotting,and RT-PCR,respectively,and then compared with 20 controlled thymus from patients carried cardiovascular surgery during the same period.The relationship of the expressions of the genes with the patient s clinical characteristics was analyzed.Re...
出处
《免疫学杂志》
CAS
CSCD
北大核心
2009年第1期83-87,共5页
Immunological Journal
