高效液相色谱法同时测定金樱子中槲皮素、山萘酚、芹菜素的含量

Simultaneousl Determined of Quercetin,Luteolin and Apigenin in Rosa laevigata by HPLC

目的建立金樱子中槲皮素、山萘酚、芹菜素含量测定方法。方法反相高效液相色谱法,色谱柱ZORBAXSB-C18(150mm×4.6mm,5μm);检测波长360nm;甲醇-0.2%磷酸(45:55)为流动相;柱温30℃;流速1.0mL/min。槲皮素、山萘酚、木犀草素、芹菜素理论板数分别大于5000、6000、6000、7000;四种化合物的分离度均大于1.5。结果槲皮素、山萘酚、木犀草素、芹菜素回归曲线分别为Y=1504.412X+9.9756,Y=1991.745X+8.6051,Y=567.591X+2.5397,Y=1811.803X+0.3074,在5.5216×10-2～19.3256×10-2μg/mL、4.608×10-2～16.128×10-2μg/mL、12.5504×10-2～43.9264×10-2μg/mL、6.0288×10-2～21.1008×10-2μg/mL范围内线性关系良好,相关系数r=0.99992～0.99998,槲皮素、山萘酚的加样回收率分别为100.5%、100.6%,RSD分别为3.7%、3.7%。样品分别含槲皮素、山萘酚、木犀草素、芹菜素0.28、0.25、0.0、0.04mg/g.结论本法为金樱子提供了分析黄酮化合物方法,该法简便可行,重复性好,数据及结果可靠。

Objective To establish a method for the determination of quercetin, luteolin and apigenin in Rosa laevigata.Metods Reverse-phase HPLC menthod was used.Results Four compounds from Rosa laevigata showed a good linear relationship, quercetin in a range of 5.521 6×10-2～19.325 6×10-2μg/mL, kaempferol was in 4.608×10-2～16.128×10-2μg/mL, luteolin was in 12.550 4×10-2～43.9264×10-2μg/mL, apigenin was in 6.028 8×10-2～21.100 8×10-2μg/mL,respectively;correlation coeff icents was 0.999 92～0.999 98.Recoveryof quercetin a...