摘要
目的优选地龙的最佳提取工艺。方法通过琼脂纤维蛋白平板法测量溶圈面积,优选出用水匀浆法作为地龙的提取方法;综合考虑得率和溶圈面积这两个指标,采用正交实验对地龙的水匀浆工艺条件进行优化。结果最佳工艺参数为:即加入5倍量水,浸渍2h,以10000r/min转速在40℃下匀浆6h。结论优选出的工艺稳定可行。
Objective To optimize the extract process of Pheretima .Methods The index of the extracting rate of Pheretima was evaluated by measuring the circular area using the fibrin-plate method.Results The optimum technology parameters are as follows:added 5 times amounts water , steeping 2 hours in water , then the homogenate for 6 hr with 10 000r/min rotation rate at 40℃ .Conclusion The optimized process is stable and highly efficient.
出处
《现代中药研究与实践》
CAS
2009年第1期58-61,共4页
Research and Practice on Chinese Medicines
关键词
地龙
提取工艺
正交实验
Pheretima
extract technology
orthogonal design
