摘要
目的:研究侯氏黑散及其拆方对大脑中动脉闭塞(MCAO)大鼠的保护作用,并探讨侯氏黑散组方配伍的意义。方法:线栓法阻断大脑中动脉建立大鼠局灶性脑缺血损伤模型,以Bederson10分制评分标准对实验大鼠作行为评分,采用HE染色对脑缺血损伤部位进行整体观察,计数海马CA1区和CA3区完整的锥体细胞;尼氏体染色结合图像分析技术检测MCAO大鼠海马锥体细胞尼氏体的变化。结果:侯氏黑散全方及其拆方君加臣药、君加臣加佐药可明显改善MCAO大鼠神经功能缺损,减轻海马神经元变性程度,增加海马锥体细胞数;侯氏黑散全方组海马锥体细胞尼氏体的脱失较模型组减少,积分光密度值显著增高。结论:侯氏黑散具有明显保护海马神经细胞的作用,全方组的疗效明显优于拆方组,符合方剂学整体取性原理。
Objectives: To study the protective role of Houshiheisan and its separate components against focal cere-bral ischemia injury in rats and discuss significance of the forming of the Houshiheisan.Methods: Cerebral ischemia injury model rats were produced by occlusion of the middle cerebral artery.Using the method of Bederson 10,evalu-ate the neurological deficiency of each group after operation.Using the method of Haematoxylin-eosin staining to ob-serve the injured location,take count of pyramidal cell in CA1 ...
出处
《山东中医药大学学报》
2009年第1期60-62,65,共4页
Journal of Shandong University of Traditional Chinese Medicine
基金
北京市教委资助项目(编号:KM2003 100025099)
首都医科大学校基金(自然科学类)(编号:20072R10)
关键词
侯氏黑散
拆方研究
脑缺血
神经保护
大鼠
Hushiheisan
formula components analysis
cerebral ischemia
neuroprotection
rat
