木犀草素-7-O-β-D-葡萄糖苷对H_2O_2诱导乳鼠心肌细胞损伤的保护作用

Protective effects of luteolin-7-O-β-D-glucopyranoside on neonatal rat cardiomyocytes injury induced by H_2O_2

目的:观察木犀草素-7-O-β-D-葡萄糖苷对过氧化氢(H2O2)诱导乳鼠心肌细胞氧化损伤的作用及其机制。方法:木犀草素-7-O-β-D-葡萄糖苷预作用12 h后,采用H2O2诱导培养乳鼠心肌细胞氧化损伤模型,测定细胞存活率、细胞内超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量、细胞内活性氧(ROS)水平及细胞内钙(Ca2+)超载的变化。结果:木犀草素-7-O-β-D-葡萄糖苷能明显提高H2O2损伤细胞存活率,提高细胞内SOD活性,降低胞内MDA和ROS生成量,同时使细胞内钙含量降低。结论:木犀草素-7-O-β-D-葡萄糖苷对H2O2氧化损伤心肌细胞具有明显的保护作用,机制可能与其抑制脂质过氧化、抑制细胞内钙超载有关。

Objective:To observe the effects of luteolin-7-O-β-D-glucopyranoside on neonatal rat cardiomyocytes injuried by hydrogen peroxide(H2O2)and study its mechanisms.Methods:After 12 hours′preconditioning of luteolin-7-O-β-D-glucopyranoside,the model of oxidative injury was induced by H2O2 in cultured neonatal rat cardiomyocytes.The viability of cardiomyocytes,superoxide dismutase(SOD)activities and intracellular malonaldehyde(MDA)levels and reactive oxygen species(ROS)activities and intracelluar calcium level we...