摘要
本实验对蕨麻中水溶性多糖进行提取和测定研究。通过单因素试验和L9(33)正交试验优化提取工艺,结果显示最佳工艺为:料液比1:30,温度100℃,时间3.5h,蕨麻多糖提取率为8.78%。同时,采用改良苯酚-硫酸法,以葡萄糖为参照品,利用紫外分光光度法测定蕨麻中多糖的含量。葡萄糖含量在10.8～75.6μg/ml范围内与吸光度呈良好的线性关系。回归方程为A=0.0124C+0.0052,R2=0.9987,得到蕨麻多糖含量为9.26%,精密度为0.829%(n=6),样品加标平均回收率为99.33%。此法操作简便,结果稳定可靠,是蕨麻多糖含量测定的有效方法。
Single factor test and orthogonal test methods L9(33) were applied to optimize the technics for extraction and determination of water-soluble polysaccharide in Potentilla anserina L. The results indicated that the optimum technics are as following: solid-liquid ratio 1:30,temperature 100 ℃ and time 3.5 h. Under these conditions,the obtained polysaccharide content is 8.78%. Using improved phenol-sulfuric acid method with glucose as the reference objective,the polysaccharide content in Potentilla anserina L. ...
出处
《食品科学》
CAS
CSCD
北大核心
2009年第2期69-73,共5页
Food Science
关键词
蕨麻
多糖
提取
测定
Potentilla anserina L.
polysaccharide
extraction
determination
