三级逆流法提取甜菊糖甙的工艺研究

Study on Three-stage Continuous Countercurrent Extraction Technology of Steviol Glycosides

本实验首先比较研究了晒干、阴干、烘干、微波干燥等几种不同的干燥方式对甜菊叶中甜菊糖甙的影响。结果表明,微波高火干燥的效果最好,只需3min,甜叶菊鲜叶中的水分从76.07%降到了3.53%且叶片颜色保持原色,莱鲍迪甙-A(Rebaudioside A,Reb-A)的含量提高了27%,甜菊苷(Stevioside,Ste)的含量提高了22%;然后比较研究了三级逆流连续提取、三罐串联逆流提取及单罐提取甜菊糖甙的提取效率,结果表明这三种方法的提取得率相近(分别为99.9%、99.82%及99.9%),但三级逆流连续提取的整个过程只需要20min,而其他两种提取方式整个过程至少需要60min,因此,三级逆流连续提取是一种更加适合于工业化大生产的提取方式。

This study aimed to develop a continuous operation mode for extracting steviol glycosides from stevia leaves instead of current batch mode by means of three-stage continuous countercurrent extraction technology. Different drying methods of stevia leaves were compared,like insolation,drying in the shade,drying in the oven and microwave drying. The results showed that microwave drying (a rapid drying method) is the best way,which only takes three minutes to dry stevia leaves from the moisture 76.07% to 3.53%,...