二极管阵列高效液相色谱法测定食品中红色2G

Determination of Red 2G in Food with HPLC-DAD

本实验建立了二极管阵列高效液相色谱法测定高蛋白食品中食品着色剂红色2G的方法。实验优化选择了高蛋白样品中红色2G的萃取溶剂、吸附剂、色谱条件。本方法应用于火腿肠中红色2G的测定,结果表明:在2mg/kg和8mg/kg两种水平上,加标回收率分别为90%～104%及89.98%～94.00%;相对标准偏差分别为9.37%和2.82%,能够满足高蛋白食品安全检测的要求。

A method for determination of food colorant Red 2G in high protein food was developed. The extractant,sorbent and separation parameters of chromatography were optimized. In this study,this method was applied to determine Red 2G in sausage. The experiment results indicated that the recovery rates for adding 2 and 8 mg/kg of Red 2G standard to sausage samples are 90% to 104% and 89.98% to 94.00% respectively,and the RSDs are 9.37% and 2.82% respectively. The method developed in this study can be applied to fo...