响应面分析法优化艾叶多糖提取工艺研究

Optimization of Extraction Technique of Polysaccharides from Argy Wormwood Leaves via Response Surface Methodology (RSM)

利用响应面法对艾叶多糖的提取工艺进行优化。在单因素试验基础上选取试验因素与水平,根据中心组合(Box-Benhnken)试验设计原理采用三因素三水平的响应面分析法,依据回归分析确定各工艺条件的影响因素,以艾叶多糖提取率为响应值作响应面和等高线。在分析各个因素的显著性和交互作用后,得出艾叶多糖浸提的最佳工艺条件为:料水比1:18、浸提温度97℃、浸提时间1.25h、浸提3次,艾叶多糖的实际一次提取率可达1.529%。

Based on single factor tests,the optimum extraction conditions of polysaccharides from argy wormwood leaves were obtained through Box-Benhnken central combination design and RSM. The results showed that the optimum extraction conditions of polysaccharides are as follows: ratio of material solvent 1:18,extraction temperature 97 ℃,extraction time 1.2 h and extraction times 3. Under such conditions,the one-time extraction rate is up to 1.529%.