禽蛋中胆固醇的酶催化分光光度测定法研究

Catalytic Spectrophotometry with Enzyme for Determination of Cholesterol in Eggs

本实验建立一种禽蛋中胆固醇含量快速测定方法——酶催化分光光度法。禽蛋匀浆液由直接皂化法处理,经酶工作液催化后,用分光光度法于波长500nm处测定其含量。方法回收率均在90%以上,变异系数均低于4%,线性范围20～600mg/ml,具有良好的准确度与精密度,适合禽蛋中胆固醇含量的快速测定。

A simple catalytic spectrophotometic with enzyme method for rapid determination of cholesterol in eggs was developed. Sample was prepared by direct saponification,and then detemined with spectrophotometry at 500 nm after enzyme catalyzed reaction. The recovery of this method is above 90% with RSD below 4% and the linear range is 20 to 600 mg/ml. It was proved that the method is precise and accurate,and so it is suitable for rapid determination of cholesterol in eggs.