期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

黏附分子CD_(44)在哮喘气道炎症反应中的作用 被引量：4

Role of CD_(44) on airway inflammatory response in rats with asthma
原文传递
导出
摘要 目的观察不同时段哮喘大鼠肺组织黏附分子CD44在哮喘气道炎症反应中的作用。方法32只Sprague-Dawley大鼠随机分成4组:哮喘模型组(激发1周组、2周组)与正常对照组(对照1周组,对照2周组),每组8只。以卵蛋白致敏制备大鼠哮喘模型,分别在激发后的1周、2周取肺组织,采用逆转录聚合酶链反应(RT-PCR)和免疫组织化学S-P方法研究肺组织CD44的表达;收集支气管肺泡灌洗液(BALF)行细胞分类计数。结果哮喘激发后1周和2周后哮喘大鼠BALF中淋巴细胞和嗜酸性粒细胞比例显著增加(P<0.05),巨噬细胞比例显著降低(P<0.05),哮喘激发后1周中性粒细胞比例也显著增加(P<0.05)。CD44在mRNA和蛋白水平的表达与BLAF中淋巴细胞、嗜酸性粒细胞呈显著正相关(均P<0.05),与激发1周时的巨噬细胞百分比呈显著负相关(P<0.05)。结论哮喘肺组织CD44的过度表达与炎性细胞向哮喘气道炎症组织趋化浸润密切相关,在哮喘气道炎症反应过程中发挥着重要作用。 Objective To study the role of cell adhesion molecule CD44 in the lung on airway inflammatory response in rats with asthma.Methods Thirty-two Sprague-Dawley rats were randomly divided into normal control and asthma groups.Asthma was induced by repeated inhalation of ovalbulium.CD44 expression in the lung was detected by semi-quantitatively reverse transcription-polymerase chain reaction(RT-PCR)and immuno-histochemical staining 1 week and 2 weeks after ovalbulium challenge.Differential leukocytes(mononuclear...
作者 李羚 杨莉 唐珩 金蕊
机构地区 南京医科大学附属无锡市儿童医院呼吸科 东南大学附属中大医院儿科
出处 《中国当代儿科杂志》 CAS CSCD 北大核心 2009年第2期142-145,共4页 Chinese Journal of Contemporary Pediatrics
关键词 哮喘 CD44 黏附分子 炎性细胞 大鼠 Asthma CD44 Cell adhesion molecule Leukocyte Rats
分类号 R562.25 [医药卫生—呼吸系统]
  • 相关文献

参考文献3

二级参考文献18

  • 1王丹琪,张冰,夏国光,赵斌,王艳霞,袁润英,王红霞,相东.过敏性支气管哮喘患者外周血T细胞亚群血清免疫球蛋白及白细胞介素－2测定[J].中华结核和呼吸杂志,1994,17(4):252-252. 被引量：10
  • 2真弓光文.アレルギ-とT细胞．サイトカイン [J].小儿科诊疗,1998,61(4):518-521.
  • 3Mark DA, Donovan CE, De Sanctis GT, He HZ, Cernadas M, Kobzik L, et al. B7-1 (CD80) and B7-2 (CD86) have complementary roles in mediating allergic pulmonary inflammation and airway hyperresponsiveness [J]. Am J Respir Cell Mol Biol, 2000, 22(3): 265-271.
  • 4Agea E, Forenza N, Piattoni S, Russano A, Monaco A, Flenghi L, et al. Expression of B7 co-stimulatory molecules and CD1a antigen by alveolar macrophages in allergic bronchial asthma [J]. Clin Exp Allergy, 1998, 28(11): 1359-1367.
  • 5Banchereau J, Bazan F, Blanchard D, Briere F, Galizzi JP, van Kooten C, et al. The CD40 antigen and its ligand [J]. Ann Rev Immunol , 1994, 12: 881-922.
  • 6Liang L, Porter EM, Sha WC. Constitutive expression of the B7h ligand for inducible costimulator on naive B cells is extinguished after activation by distinct B cell receptor and interleukin 4 receptor-mediated pathways and can be rescued by CD40 signaling [J]. J Exp Med, 2002, 196(1): 97-108.
  • 7Van Gool SW, Vermeiren J, Rafiq K, Lorr K, de Boer M, Ceuppens JL. Blocking CD40-CD154 and CD80/CD86-CD28 interactions during primary allogeneic stimulation results in T cell anergy and high IL-10 production[J]. Eur J Immunol, 1999, 29(8): 2367-2375.
  • 8Taylor PA, Friedman TM, Korngold R, Noelle RJ, Blazar BR. Tolerance induction of alloreactive T cells via ex vivo blockade of the CD40:CD40L costimulatory pathway results in the generation of a potent immune regulatory cell [J]. Blood, 2002, 99(12): 4601-4609.
  • 9Grewal IS, Flavell RA. A central role of CD40 ligand in the regulation of CD4+ T-cell responses [J]. Immunol Today, 1996, 17(9): 410-414.
  • 10Nagafuchi H, Shimoyama Y, Kashiwakura J, Takeno M, Sakane T, Suzuki N. Preferential expression of B7.2 (CD86), but not B7.1 (CD80), on B cells induced by CD40/CD40L interaction is essential for anti-DNA autoantibody production in patients with systemic lupus erythematosus [J]. Clin Exp Rheumatol, 2003, 21(1):71-77.

共引文献197

同被引文献47

  • 1马丹,张珍祥,徐永健.CD44分子的研究现状[J].国际呼吸杂志,2006,26(12):938-942. 被引量：6
  • 2胡亚美,江载芳.诸福棠实用儿科学[M].第7版.北京:人民卫生出版社,2002:1204-1205.
  • 3Thomas M, Yawn B, Price D, et al. EPOS Primary Care Guidelines: European Position Paper on the Primary Care Diagnosis and Management of Rhinosinusitis and Nasal Polyps 2007-a summary[J]. Prim Care Respir J, 2008,17 (2) : 79-89.
  • 4Lira M, Lew-Gor S, Brookes N, et al. The relationship between subjective assessment instruments in chronic rhinosinusitis[J].Rhinology, 2007,45 (2) : 144-147.
  • 5Lanza DC, Kennedy DW. Adult rhinosinusitis defined[J]. Otolaryngol Head Neck Surg, 1997,117(3) : 51-57.
  • 6Zinreich SJ. Imaging for staging of rhinosinusitis[J]. Ann Otol Rhinol Laryngol Suppl, 2004,193 : 19-23.
  • 7Borland G, Ross JA, Guy K. Forms and functions of CD44 [J]. ImmUnology, 1998,93 (2) : 139-148.
  • 8Coi T,Yamaguchi A,Nakagawara G,et al. The role of CD44 adhesion molecules [J].Nippon Rinsho, 1995, 53 (7) : 1688- 1693.
  • 9Rothenberg ME. CD44-a sticky target for asthma[J]. Clin Invest, 2003,111 (10) : 1460-1462.
  • 10Kulseth MA,Kolset SO, Ranheim T. Stimulion of serglycin and CD44 mRNA expression in endothelial cells exposed to TNF-alpha and IL-apha[J]. Bioehim Biophys Acta, 1999, 1428(2-3) : 225-232.

引证文献4

二级引证文献10

中国当代儿科杂志
2009年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部