摘要
采用从山苍子油、柠檬草油等香精油分离出的柠檬醛为材料,研究MTT比色法用于筛选抗肿瘤香精油的最佳实验条件。比较不同波长选用对吸光度测定的影响;设非挥发性抗肿瘤药物阿霉素为平行实验组,以细胞形态学变化为依据,分析了不同浓度香精油药物组、对照组在96孔板上分布对MTT法测量结果的影响;此外,还探索药物储藏温度和时间对MTT法结果重复性的影响。结果表明,选择492、570、630nm三个波长对检测结果影响甚微;气态香精油物理性位移不仅使得对照组在96孔培养板上位置直接影响实验结果,而且使得低浓度组(与高浓度相邻)细胞毒性的MTT法表征值要高于实际值:挥发性香精油于低温下贮存不仅有益于保持药效,而且能确保实验重复性。
This study focused on citral which is the main component of essential oils such as Litsea cubeba oil and lemongrass oil,and explored a experimental approach of MTT assay in the selection of antitumor essential oils.It compared the effects of different wavelengths on absorbance,taking citral as experimental drug and non-volatile adriamycin (ADM) as control,investigated their volatility and diffusibility according to morphological changes of human K562 cells,and explored the effect of stockage temperature and...
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第1期75-79,共5页
Food Science
基金
农业部重大项目(农计[2004]514)
