摘要
本实验通过土壤稀释涂布法,经过初筛、复筛,筛选出了两株酶活力较高真菌菌株C_4和G_1,在此基础上对它们生长的最佳碳源选择、酶学性质进行了研究。结果表明:菌株C_4产酶的最佳碳源为玉米芯-麸皮,培养24h就可以达到产酶高峰,其酶活力达6582.29U/ml。此酶反应最适温度为40℃,在50℃以下比较稳定,最适反应pH值为5.0,并且pH稳定性比较好;菌株G_1产酶的最佳碳源为玉米芯,培养96h就可以达到产酶高峰,其酶活力到达6301.04U/ml。此酶反应的最适温度是40℃,在40℃以下比较稳定,最适反应pH值为5.0,但pH值稳定性不如菌株C_4。
Two strains of fungi (C_4 and G_1) producing high activity xylanase were isolated by soil dilution coating method and through primary and repeated screening.Furthermore,the optimum carbon source for their growth and enzymological proper- ties of xylanases produced by them were studied.The results showed that the best carbon source for strain C_4 producing xylanase is the mixture of corncob and bran.The xylanse activity produced by strain G_4 reaches the peak after cultivation for 24h,6582. 29 U/ml.The optim...
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第1期159-163,共5页
Food Science
关键词
真菌
木聚糖酶
酶学性质
筛选
fungus
xylanase
enzymological properties
screening
