利用硅胶柱层析分离纯化平贝总碱的研究

Study on Separation and Purification of Total Alkaloids from Fritillaria ussuriensis Maxim by Silica Gel Column Chromatography

目的:对东北特产平贝母的生物碱进行分离纯化研究。方法:用硅胶柱层析法对平贝总碱进行分离纯化,固定相为薄层层析硅胶G,流动相为乙酸乙酯-甲醇-氨水(17:2:1)。用HPLC-ELSD法对分离出的贝母素甲和贝母素乙的纯度进行测定。结果:分离出贝母素甲和贝母素乙,贝母素甲纯度为93.19%、贝母素乙纯度为92.09%。结论:采用的硅胶柱层析分离纯化可得到纯度较高的分离组分。

Silica gel column chromatography was used to separate and purify total alkaloids from Fritillaria ussuriensis Maxim. The solid phase was thin layer chromatography silica gel G and the mobile phase was ethyl acetate-methanol-ammonia (17:2:1). The purities of the separated products,pemine and peinine were determined using HPLC-ELSD.They are 93.19% and 92.09%, respectively.In conclusion,the separation method of silica gel column chromatography applied in this experiment can be used to gain highly purified prod...