摘要
潮霉素磷酸转移酶基因广泛用于转基因生物的基因转化过程中,该基因(hpt)编码的蛋白属于无食用和食物过敏史的处源目的蛋白。根据IFBC-ILSI制定的转基因食品致过敏性评价方法,进行了与已知的过敏蛋白的氨基酸序列相似性比较、消化稳定性实验以及动物模型实验。结果表明,未发现连续8个氨基酸序列相同:该蛋白在80℃加热10min左右时已全部降解,不具有热稳定性:体外模拟消化实验表明,消化20min时,SDS-PAGE和Western Blotting已检测不出Hpt蛋白。实验采用BN大鼠作为过敏模型,ELISA检测特异IgG、IgE以及组胺水平,Hpt蛋白与阳性存在显著性差异(p<0.05),与阴性无显著性差异。综合结果表明,Hpt蛋白潜在致敏性很低。本研究可为进一步开展以hpt基因为标记性基因的作物上市前安全性的评价提供基础数据。
Hygromycin B phosphotransferase gene (hpt) has been widely used in the process of gene construction when agricultural plants are modified by biotechnology.Hpt protein as an introduced"new"protein was obtained from sources with no prior history of causing allergy,thus its safety assessment was mainly focused on comparison of amino acid sequences, in vitro digestibility and animal model according to the IFBC-ILSI.Individual sequence analysis with known amino acid sequences, reported as allergens,showed that n...
出处
《食品科学》
CAS
CSCD
北大核心
2009年第1期261-264,共4页
Food Science
基金
国家“863”计划项目(2006AA10Z440)
农业部“948”项目(2007-Z8)
关键词
潮霉素磷酸转移酶
安全评价
体外消化
动物模型
过敏
hygromycin Bphosphotransferase gene
safety assessment
in vitro digestibility
animal model
allergenicity
