药用植物功能基因克隆新方法——成分差异表型克隆法

New method of "ingredient difference phonetypical cloning" for functional gene cloning from medicinal plants

针对目前大多数药用植物遗传背景薄弱,有效成分复杂,一般克隆方法难以获得有效成分生物合成相关功能基因的困境,笔者提出并实践了"成分差异表型克隆法",介绍了其概念、原理及方法,通过实例验证了该方法的可行性。指出该方法尤其在有效成分次生代谢途径不明确、相关功能基因背景信息未知的情况下,进行功能基因克隆具有比较突出的优势。为中药功能基因克隆研究探索了新的研究思路和方法,将为深入开展药用植物基因工程、道地药材形成机制等研究奠定基础。

This paper introduced a new method of "ingredient difference phonetypical cloning" for functional gene clone of medicinal plants,which might solve the difficulties in isolating genes encoding enzymes for the biosynthesis of secondary metabolites by usual ways. Concepts,mechanisms and methods were systematically introduced and possibility was proved by experiments. The method showed the extra superiority of for the isolation of the genes belonged to unknown metabolic pathway and little information about its ...