川芎嗪对肾间质纤维化模型大鼠Smad7和SnoN蛋白表达的影响

Effect of tetramethylpyrazine on expression of Smad7 and SnoN in rats with UUO

目的:探讨川芎嗪(tetramethylpyrazine,TMP)对单侧输尿管梗阻(unilateral ureteral obstruction,UUO)所致大鼠肾间质纤维化的作用及机制。方法:雄性SD大鼠18只,随机分为3组:假手术组、模型组以及TMP组,每组6只。采用单侧输尿管结扎建立大鼠肾间质纤维化模型。川芎嗪(40mg·kg-1·d-1)治疗3周后取梗阻侧肾组织,分别进行HE和Masson染色,观察肾组织病理改变和胶原沉积的变化;采用放射免疫分析法检测Ⅲ型前胶原含量;应用酶联免疫吸附(ELISA)法检测肾组织中转化生长因子-β1(transforming growth factor-β1,TGF-β1)水平;采用Western blotting检测肾组织中Smad7和Smad转录共抑制因子SnoN蛋白表达水平的变化。结果:与假手术组比较,镜下可见模型组大鼠肾小管萎缩,管腔扩张,肾间质中大量胶原纤维沉积,肾间质明显增宽;Ⅲ型前胶原、TGF-β1含量显著增加;Smad7,SnoN蛋白表达水平显著下调。TMP组与模型组比较,肾组织病理改变明显改善,Ⅲ型前胶原、TGF-β1含量明显降低,Smad7,SnoN蛋白表达水平明显上调。结论:川芎嗪具有显著抗肾间质纤维化的作用,其机制与降低肾组织中促纤维化细胞因子TGF-β1含量,同时恢复Smad逆向调控因子Smad7和SnoN蛋白表达水平有关。

Objective:To study effect and mechanisms of tetramethylpyrazine(TMP) on renal interstitial fibrosis induced by unilateral ureteral obstruction in rats. Method:Eighteen male sprague-dawley rats were randomly divided into sham-operated group,model group and TMP group,6 in each group. The model of renal interstitial fibrosisi was established in rats by unilateral ureteral obstruction. All rats were killed at the end of the 3th week after treatment. Pathological change and collagen deposition of the kidneys in ...