大黄抑制鼠疫耶尔森菌分子机制的初步研究

Study of molecular mechanism of Rheum offcinale against Yersinia pestis

目的:探索大黄抑制鼠疫耶尔森菌作用的分子机制。方法:液体稀释法测定大黄水提物对鼠疫菌生长抑制作用的最小浓度(MIC)。应用鼠疫耶尔森菌全基因组芯片研究大黄作用鼠疫菌的表达谱。选择10倍MIC做为大黄抑制鼠疫菌作用浓度,作用时间分别选择30,60min。提取并纯化鼠疫耶尔森菌总RNA;逆转录合成cDNA;用Cy3,Cy5染料标记后,与鼠疫耶尔森菌全基因组芯片杂交;通过芯片扫描仪获得表达谱分析结果。应用SAM软件对结果进行分析;应用RT-PCR对芯片结果进行验证。结果:得到了大黄不同作用时间鼠疫菌的表达谱。获得了大黄不同作用时间鼠疫菌的相同差异表达基因。结论:大黄抑制鼠疫菌的主要分子机制在于影响细菌核糖体蛋白质合成相关基因与细胞膜相关基因的表达。

To investigate molecular mechanism of traditional Chinese medicine Rheum offcinale against Yersinia pestis,whole genome DNA microarray that contains 4005 annotated genes of Y. pestis was used. The minimal inhibition concentration(MIC) of R. offcinale extract against Y. pestis was determined by liquid dilution method. The gene expression profile of Y. pestis was performed after exposured to R. offcinale extract at a concentration of 10×MIC for 30 and 60 minutes. The total RNA extracted and purified from Y. p...