中药九节茶提取物对应激负荷小鼠免疫功能的改善作用

Effects of Sarcandra glabra extract on immune activity in restraint stress mice

目的:研究中药九节茶对束缚应激小鼠免疫功能的改善作用。方法:实验将雄性C57BL/6小鼠分为正常对照组,应激对照组,125,500mg·kg-1九节茶提取物给药组,制备脾脏淋巴细胞悬液,流式细胞术分析T淋巴细胞亚群、NK细胞和NKT细胞比例及数目。结果:与应激对照组相比,中药九节茶可以提高束缚应激小鼠脾脏淋巴细胞总数,改善T淋巴细胞亚群比例,同时增加脾脏NK细胞和NKT细胞比例及数目。结论:中药九节茶可以改善应激负荷小鼠淋巴细胞数目及淋巴细胞亚群比例。

Objective:To study the protective effect of Sarcandra glabra extract(SGE) on immune system in restrained mice. Method:The male C57BL/6 mice were randomly divided into normal control group,stress control group,125,500 mg·kg-1 SGE group. The spleen lymphocyte suspensions of each group were prepared. The parameters of spleen T cells subsets,NK cell and NKT cell proportion and number was detected by Flow cytometry. Result:SGE regulated the balance of T cell subsets,increased the percent of NK cells and NKT cell...