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用合成线性多肽研究GD小鼠模型的免疫状态 被引量：2

The immune responses in Graves' disease mouse model as investigated through synthetic linear peptides
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摘要 用合成的线性多肽探讨Graves病(Graves disease,GD)小鼠模型的免疫状态。用表达促甲状腺素受体(TSHR)A亚单位的腺病毒(A-TSHR-Ad)免疫BALB/c雌性小鼠获得GD小鼠模型;用合成的TSHR A亚单位的线性多肽PA,刺激小鼠脾脏细胞,检测记忆性淋巴细胞反应中生成的细胞因子。所有接受免疫的小鼠均出现TRAb增高,66.7%的小鼠出现甲亢;接受免疫的小鼠脾脏细胞在接受多肽刺激后,IFN-γ生成增加(PPA-2=0.01),IL-4的生成则未受影响。表明T细胞可以识别TSHR A亚单位的线性多肽PA,多肽PA可以促进GD小鼠Th1免疫应答的发生。 To investigate the immune responses in Graves disease(GD) mouse model with synthetic linear peptides,the female BALB/c mice were immunized with adenovirus expressing the A subunit of TSH receptor(TSHR)(A-TSHR-Ad),thus producing the GD mouse model.The splenocytes of this mouse model were stimulated with synthetic linear peptides,and the cytokines produced in the culture supernatants of the stimulated memory lymphocytes were assayed.It was found that all the immunized mice showed an elevated level of TRAb wi...
作者 赵泽飞 王曙 崔九兰 崔斌 赵咏桔 宁光
机构地区 上海交通大学医学院附属瑞金医院内分泌代谢科上海市内分泌代谢病临床医学中心上海市内分泌代谢病研究所
出处 《现代免疫学》 CAS CSCD 北大核心 2009年第1期55-58,共4页 Current Immunology
基金 上海市重点(优势)学科资助项目(Y0204) 上海市卫生局科研基金资助项目(044054)
关键词 GRAVES病 小鼠模型 线性多肽 Γ干扰素 Graves disease mice models linear epitopes interferon-γ
分类号 R392.9 [医药卫生—免疫学]
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二级参考文献10

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共引文献4

同被引文献24

  • 1赵泽飞,王曙,崔九兰,顾雪疆,唐金凤,赵咏桔.表达TSHR-A亚单位的腺病毒免疫BALB/c雌性小鼠建立甲亢模型[J].现代免疫学,2008,28(3):229-232. 被引量：5
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  • 6Chang YH, Yang JS, Yang JL, et al. Gandoderma lucidum extract promotes immune responses in normal BALB/c mice in vivo [ J ]. Vaccine. 2009. 23 (5) : 755 - 759.
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  • 8Saitoh O, Mizutori Y, Takamura N, et al. Adenovirus-mediated gene delivery of interleukin-lO, but not transforming growth factor beta, ameliorates the induction of Graves' hyperthyroidism in BALB/c mice[J]. ClinExp Immunol, 2005, 141(3): 405 -411.
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现代免疫学
2009年 第1期

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