摘要
建立双抗体夹心酶联免疫吸附法(ELISA)检测血清中骨膜素水平及初步应用于临床消化系肿瘤的检测。采用ELISA技术,利用双抗体夹心法制备骨膜素检测试剂盒,并进行自制试剂盒的指标评价。结果:双抗夹心ELISA法检测骨膜素的最小检出限为4.803 ng/ml,标准曲线在(4.803~500)ng/ml范围内线性良好。平均批内变异系数为7.2%,平均批间变异系数为14.1%,回收率为84.5%。278例胃肠道腺癌患者的血清骨膜素浓度为(40.9±15.4)ng/ml,显著高于63名健康对照者[(21.0±7.3)ng/ml,P<0.0001]和56例胃肠道腺瘤或息肉患者[(22.4±8.5)ng/ml,P<0.0001]。骨膜素ELISA试剂盒具有良好的灵敏性、特异性和准确性,今后有望用于临床消化系肿瘤的检测。
To develope double antibodies sandwich-enzyme linked immunosorbent assay for detecting periostin in serum and to evaluate its preliminary clinical application for cases with gastrointestinal carcinoma.A sandwich-ELISA diagnostic kit was developed by using anti-periostin monoclonal antibody and all parameters of the kit were evaluated.It was found that the lowest limit of detection was 4.803 ng/ml;and the linearity of the standard curve was preferable from 4.803 ng/ml to 500 ng/ml.The average intra-assay and...
出处
《现代免疫学》
CAS
CSCD
北大核心
2009年第1期64-67,共4页
Current Immunology
关键词
骨膜素
消化系肿瘤
酶联免疫吸附检测
试剂盒
periostin
gastrointestinal carcinoma
enzyme linked immunosorbent assay
reagent kits
