摘要
Objective This study aimed to investigate the possible role of Orphanin FQ(OFQ) in the regulation of hypothalamic gonadotropin-releasing hormone(GnRH) secretion. Methods The method of push-pull perfusion and radioimmunoassay(RIA) were adopted to examine the secretory profile of GnRH in the median eminence(ME) in freely moving ovariectomized(OVX) rats after intracerebroventricular(icv) injection of OFQ and/or [Nphe1]NC(1-13)NH2(NC13),a competitive antagonists of the opioid receptor-like 1 receptor(ORL1 recep...
目的本文旨在研究孤啡肽(Orphanin FQ,OFQ)对下丘脑促性腺激素释放激素(gonadotropin-releasing hormone,GnRH)释放的调节机制。方法应用推挽灌流和放射性免疫测定方法观察侧脑室注射OFQ和/或孤儿受体(opioidreceptor-like1receptor,ORL1receptor)特异性拮抗剂NC13对清醒去卵巢(ovariectomized,OVX)大鼠下丘脑正中隆起(median eminence,ME)GnRH释放的影响。结果侧脑室给予20和200nmol孤啡肽,注射后40到80min可明显抑制OVX大鼠ME的GnRH释放(P<0.05),该作用可被侧脑室注射等剂量NC13预处理所阻断。侧脑室单独给予100和200nmol NC13,注射后60min可使OVX大鼠ME的GnRH释放显著增加(P<0.05)。结论中枢注射OFQ通过ORL1受体可抑制OVX大鼠ME的GnRH释放,提示下丘脑OFQ可能参与了GnRH释放的生理调节机制。
基金
supported by the National Natural Science Foundation of China (No. 39870914)
the Key Project on Traditional Chinese Medicine of Shanghai Medical Health Bureau (No. 20003).
