Initial bradykinin triggers calcium-induced calcium release in C6 glioma cells and its significance

缓激肽触发C6胶质瘤细胞内Ca^(2+)内流诱发的Ca^(2+)释放(英文)

Objective To investigate the underlying mechanism for the selective modulation of the permeability of blood-tumor barrier(BTB) by small dose of bradykinin(BK). Methods C6 glioma cells were treated with BK,and changes of intracellular nitric oxide(NO) and intracellular calcium level were measured with fluorescent spectrophotometer. Results The initial application of BK easily triggered extracellular calcium influx,which resulted in intracellular calcium store release in C6 glioma cells. The above mechanism w...

目的探讨小剂量缓激肽选择性开放血肿瘤屏障的内在机制。方法通过免疫荧光探针实时测定缓激肽刺激前后C6胶质瘤细胞内的钙离子和一氧化氮(nitric oxide,NO)的变化。结果C6胶质瘤细胞上存在尼诺丁敏感受体,小剂量缓激肽可以引起肿瘤细胞外的钙离子内流,导致细胞内钙离子水平升高,特别是首次刺激后,细胞外钙离子内流可以选择性的引起C6胶质瘤细胞内的钙库释放,即Ca2+内流诱发的Ca2+释放(calcium-induced calcium release,CICR)。同时,缓激肽刺激后,在C6胶质瘤细胞内存在NO的持续升高,且NO是继发于细胞内尼诺丁受体介导的CICR之后。结论小剂量缓激肽选择性触发C6胶质瘤细胞内钙库释放,可能是小剂量缓激肽可以选择性开放血肿瘤屏障的原因。