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增强型绿色荧光蛋白在集胞藻6803中的表达 被引量：1

Expression of Enhanced Green Fluorescent Protein (eGFP) in Synechocystis sp. PCC6803
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摘要 利用聚球藻7942热休克基因groESL的启动子和报告基因egfp,构建了表达载体pUC-Tegfp并转化集胞藻6803,并通过所制备抗体对转基因藻进行蛋白免疫印迹检测.结果发现,在转基因藻株T-egfp的细胞粗提液中含有能与eGFP抗体特异结合的蛋白质,表明外源增强型绿色荧光蛋白基因(egfp)在集胞藻6803中成功表达. The groESL gene promoter,originated from Synechococcus sp.PCC 7942,and the reporter gene egfp coding region were ligated into pUC19 vector to generate the shuttle expression plasmid,pUC-Tegfp,which was subsequently transferred into Synechocystis sp.PCC6803.Immunoblotting analysis showed the presence of eGFP protein in transgenic cells harboring pUC-Tegfp.This indicated that eGFP protein was cloned and overexpressed in transgenic cells.
作者 陈伟伟 郭正红 康升云 魏兰珍 王全喜
机构地区 上海师范大学生命与环境科学学院
出处 《西北植物学报》 CAS CSCD 北大核心 2009年第2期229-233,共5页 Acta Botanica Boreali-Occidentalia Sinica
基金 上海市高等学校科技基金重点项目(05ZZ15) 上海师范大学校基金(SK200837)
关键词 集胞藻6803 增强型绿色荧光蛋白 转基因藻 Synechocystis sp.PCC6803 eGFP transgenic Synechocystis sp.PCC6803 harboring pUC-Tegfp
分类号 Q943.2 [生物学—植物学]
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参考文献2

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同被引文献23

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西北植物学报
2009年 第2期

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