不同采收期珊瑚菜中香豆素含量的比较

Comparision on the Content of Coumarin in Glehnia littoralis during Different Harvest Periods

应用高效液相色谱技术,分析了当年生珊瑚菜栽培后期4个采收时期的根、叶以及采后根去皮处理对香豆素含量的影响.结果显示:(1)珊瑚菜的根中,香豆素的含量和产量在10月15日均达到最高,分别为0.0772mg?g-1和1.0878mg/株,且显著高于其他采收期,其中,补骨脂素在栽培后期含量相差不大,欧前胡素和异欧前胡素均在10月15日含量最高.(2)珊瑚菜的叶中,不同采收期均有一定量的香豆素存在,其中在9月15日采收时,香豆素的含量和产量均极显著高于其他采收期,分别达到0.2065mg?g-1和1.3175mg/株,为同期根中香豆素含量和产量的4.4倍和2.0倍.(3)珊瑚菜根经水烫去皮处理后香豆素总量急剧下降到0.0081mg?g-1,与同期采收未去皮处理的根相比,去皮后根内香豆素总含量、补骨脂素、欧前胡素、异欧前胡素含量分别下降了87.7%、100%、82.76%、85.25%.(4)9月15日采收的珊瑚菜单株(根+叶)香豆素总产量(根+叶)极显著高于其他采收期,达1.9802mg/株,其次为10月15日1.1575mg/株.研究表明:珊瑚菜叶应作为提取香豆素的重要原材料,采收时应一起收获;对珊瑚菜根去皮处理会导致根的药用质量大幅度下降,生产中应明确禁止;珊瑚菜的采收期应依据其用途决定,以根直接入药应选择10月15日采收,作为提取香豆素生产原料应选择9月15日采收.

The content change of three kinds of coumarin(psoralen,imperatorin and isoimperatorin)in roots and leaves of Glehnia littoralis in four stages of late cultivation periods were analyzed by HPLC,while those in roots after two treatments of being peeled off and not after harvest were also analyzed.The results were as follows:(1)Both of the highest yield of coumarin(1.087 8 mg/strain)and the highest content of coumarin(0.077 2 mg·g-1)appeared in the mid-October in the roots of G.littoralis.They were significant...