棉纤维发育相关基因GhCHS、GhCPI的克隆与鉴定

Cloning and Characterization of GhCHS and GhCPI Which Related with Fiber Development in Gossypium hirsutum L.

以陆地棉李氏超短纤维突变体Li1li1自交后代野生型li1li1和超短纤维突变体Li1li1开花后4 d的胚珠纤维的复合体的RNA为探针,通过基因芯片的方法筛选优质材料7235棉纤维伸长、次生壁加厚不同发育时期混合cDNA文库,分离出两个在两种材料中差异表达的cDNA序列,分别命名为GhCHS(GenBank登录号:EF643506)和GhCPI(GenBank登录号:EF643507)。RT-PCR分析表明:开花后4 d,GhCHS,GhCPI在陆地棉李氏野生型li1li1纤维中的表达量低于超短纤维突变体Li1li1。Southern杂交结果表明两个基因在陆地棉基因组中都存在两个拷贝。

To isolate genes preferentially expressed during fiber elongation period,genechips were performed with wild line li1li1 and the Ligon lintless mutant line Li1li1 segregated from the self-crossing progenies of the Ligon lintless mutant line Li1li1.Two differently expressed cDNA clones were separated from cotton fiber library of elite quality material 7235 during fiber elongation and the second wall synthesis by genechips with probes of the 4 DPA ovule-fiber compound from the wild line li1li1 and the Ligon li...