摘要
目的通过建立小鼠哮喘模型,以卡介苗(BCG)和结核菌素纯蛋白衍生物(PPD)作干预因素,从而了解哮喘小鼠CD4+ CD25+调节性T细胞和其它T细胞亚群数量的变化,以及肺部的病理改变。方法将36只6～8周龄的BALB/C小鼠随机分成A、B、C、D4组,A组为BCG+BCG干预组,B组为BCG+PPD干预组,C组为哮喘模型组,每组各10只,D组6只为正常对照组。应用流式细胞仪分别检测各组的CD4+ CD25+调节性T细胞和T细胞亚群,同时取各组的肺组织PAS染色鉴定黏液分泌。结果A、B、C三组的CD3+、CD4+、CD8+ T细胞的百分比以及CD4+/CD8+的比值均比D组(正常对照组)低(P<0.05),而A、B两组的CD3+T细胞百分比较C组增高(P<0.05)。CD4+ CD25+ Treg占CD4+T细胞的百分比,其它三组低于D组(P<0.05),A、B两组较C组升高(P<0.05),而A、B两组之间的差异无统计学意义(P>0.05)。肺组织病理提示D组未见到黏液分泌;C组支气管壁可见杯状细胞增生、变大,并有大量黏液分泌;A、B两组与C组比较,杯状细胞减少,黏液分泌减少。B组气道的炎症反应轻于A组。结论支气管哮喘的发病可能与CD4+ CD25+ Treg数量的减少及T细胞亚群功能紊乱有一定的关系;卡介苗单独应用或与PPD联合应用都能够在一定程度上减轻哮喘小鼠气道的黏液分泌,并可以提高CD4+ CD25+ Treg数量;BCG+PPD联合应用较BCG+BCG应用对哮喘的预防效果更明显。
Objective This study examined the effect of BCG vaccination and PPD on CD4+ CD25+ regulatory T cells in asthmatic mice and its preventive effect on airway inflammation.Methods 36 BALB/C mice were divided into four (4) groups (group A, B, C and D) randomly. There were 10 mice in group A, B and C respectively,and there were six (6) mice in group D. The mice of group A, B, and C were sensitized and challenged with ovalbumin (OVA) to establish asthmatic models. Mice in Group A were injected with BCG twice, that...
出处
《安徽医学》
2009年第3期215-218,共4页
Anhui Medical Journal
基金
安徽省教育厅资助课题(kj2008A1)
