K-ras基因突变和p21蛋白的表达与子宫内膜异位症恶性转化的关系

The association of K-ras mutations and p21 expressions with malignant transformation of endometriosis

目的:探讨K-ras基因点突变和p21蛋白的表达与子宫内膜异位症(内异症)恶性转化的关系。方法:应用PCR-RFLP和免疫组织化学技术,对与内异症相关的3例卵巢子宫内膜样癌及3例卵巢透明细胞癌中K-ras基因第一外显子第12、13密码子的突变情况以及p21蛋白的表达进行研究,并将结果与和内异症不相关的卵巢子宫内膜样癌、卵巢透明细胞癌以及不典型内异症、卵巢内异症相比较。结果:起源于内异症的卵巢子宫内膜样癌和卵巢透明细胞癌中各有1例检测到K-ras基因第12密码子发生突变,突变类型为GGT→TGT,即甘氨酸→半胱氨酸,其余标本均未见第12、13密码子突变。p21蛋白在与内异症相关的卵巢肿瘤中的表达高于其他各组(P<0.05)。结论:K-ras基因的改变与内异症恶性转化可能具有某种联系。p21蛋白在与内异症相关的卵巢癌中表达较高,说明它们的发病可能涉及不同的分子机制。

Objective To explore the association of K-ras mutations and p21 expressions with malignant transformation of endometriosis.Methods K-ras mutations in codons 12 and 13 and expressions of p21 protein were detected in 3 endometriosis-related ovarian endometrioid carcinomas and in 3 endometriosis-related ovarian clear cell carcinomas using PCR-RFLP and immunohisochemical technique.The findings were compared with those from ovarian carcinomas unrelated to endometriosis, atypical endometriosis, and ovarian endome...