REG1a基因单核苷酸多态性与胃癌遗传易感性研究

Association of Single Nucleotide Polymorphisms of REG1a Gene and Genetic Susceptibility of Gastric Carcinoma

目的寻找中国汉族人群REG1a基因单核苷酸多态性位点,探讨其基因多态性与胃癌(Gastric Cancer)的关系。方法应用PCR产物测序的方法在中国人群中检测散发性胃癌组(n=183)及对照组(n=204)REG1a基因的单核苷酸多态性(single nucleotide polymorphisms,SNPs)。结果REG1a基因第929位(T/C)、第1790位(C/G)、第2751位(A/T)发现3个新的SNP位点,其中第929位和第1790位2个位点基因型在胃癌与健康人群的差异具有统计学意义(P<0.05)。结论对特定人群进行测序可有效筛查出候选SNP位点,初步发现REG1a基因的2处单核苷酸多态性与胃癌的发生、淋巴转移和远处转移有一定关系,为后续利用单核苷酸多态性标志进行胃癌风险预测奠定了研究基础。

Objective Toinvestigate the association between genetic susceptibility of gastric carcinoma and single nucleotide polymorphism of REG1a gene in Chinese Han population.Methods The genotype of REG1a gene were detected by PCR and DNA sequencing in 183 Chinese patients with gastric carcinoma and 204 controls.Results It was found 3 new SNPs in REG1a gene at site 929(T/C),1790(C/G) and 2751(A/T),respectively;and the different genotypes of site 929 and 1790 were significant between gastric carcinoma patients and c...