摘要
目的观察高糖培养条件下离体雪旺细胞活力和增殖能力的变化以及中药红景天提取物烯苷对该变化的影响,探讨糖尿病性周围神经损伤和药物作用的可能机制。方法采用Bockes改良法从新生Wistar乳鼠坐骨神经组织分离纯化雪旺细胞;采用XTT法测定雪旺细胞活力:采用3H-TdR掺入法检测雪旺细胞的增殖能力。结果高糖对离体雪旺细胞活力和增殖能力具有显著抑制作用;烯苷对高糖导致雪旺细胞增殖能力的抑制性变化具有显著的改善作用。结论烯苷改善高糖条件下离体雪旺细胞增殖能力的抑制性变化,为中药红景天临床治疗糖尿病性周围神经损伤提供了进一步的实验依据。
Objective To observe changes in activity and proliferation of cultured Schwann cells under the high glucose condition and effect of allyl glycoside extracted from Herba Rhodiolae(AG) on these changes for exploring the possible mechanism of diabetic peripheral nerve injury and the pharmacologic action of AG on them.MethodsAdopting refined Brokes method,Schwann cells were isolated from the sciatic nerve tissue of newborn Wistar rats and purified.Their activity and proliferative capability were determined usin...
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2009年第2期153-155,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
上海市科委重点项目资金资助(No.014319321)
关键词
糖尿病周围神经病变
雪旺细胞
高糖
烯苷
diabetic peripheral neuropathy
Schwann cells
high glucose
allyl glycoside
