内生拮抗细菌BEB2的分子鉴定及其对香蕉枯萎病菌的抑制作用

Identification of Endophytic Bacillus and Its Inhibition Reaction to F. oxysporum f. sp. cubense of Banana

利用聚合酶链式反应(PCR)方法对细菌BEB2做进一步的分子鉴定,并研究不同温度、pH值和培养基下BEB2发酵液对香蕉枯萎病菌生长的抑制能力,以及应用BEB2对香蕉枯萎病进行防治测试。结果发现,香蕉内生拮抗细菌BEB2为枯草芽孢杆菌(Bacillus subtilis);不同温度、pH值和培养基下的BEB2发酵液对香蕉枯萎病菌生长的抑制能力具显著差异,在King B2培养基上抑菌作用最强,最适抑菌温度为32℃,最适抑菌pH值为9.0。香蕉枯萎病盆栽防治实验结果发现,菌株接种离体叶片和植株均表现出一定的抑制活性,防治效果良好;该菌株连续20次转接培养,遗传稳定性高,抑菌谱较宽,具一定的生防应用价值。

The strain BEB2 of endophytic Bacillus isolated from banana was identified as Bacillus subtilis according to its homology analysis of 16S rDNA sequence.The temperature,pH and medium had a significant impact to the inhibition of BEB2 to the banana wilt disease.The inhibitory effect was strongest in King B2 medium,the optimum temperature for the highest antimicrobial activity was 33℃,and the optimal pH9.0.In the antagonistic test of BEB2 to the banana wilt disease,the inhibition was obvious with different in ...