辛伐他汀对炎症损伤后血管内皮祖细胞的保护作用

Protective effect of simvastatin on inflammatory injury of EPCs

目的探讨肿瘤坏死因子α(TNF-α)对血管内皮祖细胞(EPCs)产生E-选择素和内皮型一氧化氮合酶(eNOS)活力的影响及不同浓度辛伐他汀的干预作用。方法体外培养与鉴定兔骨髓源EPCs,用不同浓度TNF-α(1、10、25、50 ng/ml)刺激24 h;50 ng/ml TNF-α刺激细胞0.5 h后用不同浓度辛伐他汀(0、0.1、1.0、10μmol/L)干预24 h,收集上清;用ELISA法检测可溶性E-选择素的浓度,用化学比色法测定eNOS活力。结果在TNF-α刺激下EPCs产生E-选择素增加,同时eNOS活力降低(P<0.05),变化的量和TNF-α的浓度呈相关性;加入辛伐他汀后E-选择素明显下降,eNOS活力与辛伐他汀呈明显的浓度依赖性增大(P<0.05)。结论辛伐他汀对于炎症损伤后的EPCs具有保护作用。

Objective To investigate the effect of tumor necrosis factor(TNF)-α on E-selectin secretion and endothelial nitric oxide synthetase(eNOS) activity of endothelial progenitor cells(EPCs),and simvastatin interference on them.Methods EPCs cultured in vitro were stimulated with TNF-α(1,10,25,50 ng/ml)for 24 h.After stimulated by TNF-α(50 ng/ml) for 30 min,the EPCs were intervented by different concentrations(0,0.1,1.0,10 μmol/L) of simvastatin for 24 h.Soluble E-selectin in the supernatant was detected by ELISA ...