摘要
目的探讨胰岛素抵抗大鼠丝氨酸/酪氨酸磷酸化异常与脂联素(APN)的关系。方法高脂饲料喂养10周建立胰岛素抵抗大鼠模型,并用正常血糖-高血浆胰岛素钳夹技术评估。应用ELISA法检测大鼠血清APN含量,Westernblot法检测肝脏组织中胰岛素受体底物1(IRS-1)磷酸化丝氨酸(IRS-1Ser636)及磷酸化酪氨酸(IRS-1Tyr465)表达。结果高脂组大鼠葡萄糖输注率(GIR60～120)明显低于基础饲料组(1.56±0.43vs5.15±0.66mg·kg-1·min-1,P<0.01);与基础饲料组相比,高脂饲料组大鼠血清APN水平显著降低(0.70±0.07vs0.85±0.12pg/ml,P<0.01),IRS-1Tyr465表达显著降低(111.68±13.41vs127.77±13.17,P<0.05),IRS-1Ser636水平显著升高(109.47±13.75vs94.23±15.05,P<0.05)。血清APN水平与IRS-1Tyr465正相关(r=0.68,P<0.01),与IRS-1Ser636负相关(r=-0.70,P<0.01)。结论胰岛素抵抗大鼠APN水平与IRS-1Tyr465正相关,与IRS-1Ser636负相关,提示APN改善胰岛素抵抗机制可能与纠正IRS-1磷酸化异常有关。
Objective To investigate the relationship between adiponectin and abnormality of insulin receptor substrate-1(IRS-1)phosphorylation in insulin-resistant rats.Methods Insulin resistant rats model were established by high-fat diet feeding.Thirty male Wistar rats were randomly divided into 2 groups,each fed with either basic chow(NC,n =15)or high-fat diet(HF,n =15)for 10 weeks,then assessed by euglycemie-hyperinsulinemia clamp technique.Adiponectin was determined with ELISA and phosphorylation of IRS-1 was mea...
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2009年第4期250-252,共3页
Journal of China Medical University
基金
辽宁省教育厅高校科研基金资助项目(2008765)
关键词
胰岛素抵抗
大鼠
丝氨酸磷酸化
酪氨酸磷酸化
脂联素
insulin resistance
rat
serine phosphorylation
tyrosine phosphorylation
adiponectin
