玉米籽皮稀酸水解液脱毒发酵制备丁二酸的可行性

Feasibility for producing succinic acid by fermentation of cornhusk dilute acid hydrolysate after detoxification

丁二酸是一种重要的碳四平台化合物,利用非粮生物质替代淀粉制备丁二酸可保证粮食安全并降低原料成本。玉米籽皮是一种廉价的非粮生物质,该文采用稀酸水解玉米籽皮制备混合糖液,并对玉米籽皮稀酸水解液的脱毒条件进行了优化,优化结果为:活性炭用量1%(m/V)、pH4.0、作用温度30℃、作用时间30 min。在此工艺条件下,水解液的脱色率为92.27%,糠醛脱除率75%,5-羟甲基糠醛脱除率53%,多酚类化合物脱除率98%,总糖损失低于5%。经过脱毒处理后,产琥珀酸放线杆菌NJ113均能利用水解液中的葡萄糖、木糖、阿拉伯糖,培养基总糖浓度为50 g/L时,丁二酸分批发酵的质量收率可达0.68 g/g,浓度可达34.2 g/L,生产强度达0.83 g/(L·h),总糖浓度为68.2 g/L时,丁二酸质量收率仍可达0.62 g/g,浓度42.3 g/L,生产强度0.98 g/(L·h)。发酵试验表明脱毒的玉米籽皮稀酸水解液作为碳源厌氧发酵制备丁二酸具有可行性。

Succinic acid is an important C4 platform chemical.Utilization of non-food biomass instead of starch for succinic acid production by fermentation does not threaten grain provisionment and can reduce the cost of substrate. Cornhusk is a kind of non-food and low-cost biomass.Cornhusk was hydrolyzed by dilute acid in this research. Optimized technical conditions for detoxification of cornhusk dilute acid hydrolysate were determined by experiments: activated carbon 1%(W/V),pH4.0,temperature 30℃,detoxification t...