RP-HPLC测定芫花中山柰酚-3-O-β-D-(6″-p-香豆酰)-吡喃葡萄糖苷的含量

RP-HPLC determination of tiliroside in Daphne genkwa

目的:对芫花中山柰酚-3-O-β-D-(6″-p-香豆酰)-吡喃葡萄糖苷(tiliroside)的含量进行测定。方法:采用反相高效液相色谱,ZORBAX XDB C18色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈(A)-0.1%醋酸水溶液(B),梯度洗脱,0～18 min A:B 26∶74,18～33 mim A:B 80∶20;流速1.0 mL.min-1,检测波长310 nm,柱温25℃。结果:山柰酚-3-O-β-D-(6″-p-香豆酰)-吡喃葡萄糖苷在进样量0.1~5.0μg与峰面积呈良好的线性关系,r=0.999 9(n=6)。山柰酚-3-O-β-D-(6″-P-香豆酰)-吡喃葡萄糖苷的平均回收率101.50%,RSD 0.90%(n=6)。结论:该方法可准确地测定芫花中山柰酚-3-O-β-D-(6″-p-香豆酰)-吡喃葡萄糖苷,方法精密度高、简便、快速。

Objective:To determine the contents of tiliroside [kaempferol 3-O-β-D-(6'-p-coumaroyl) glucopyranoside] in the Daphne genkwa.Method:The separation was performed on ZORBAX XDB C18 column(4.6 mm×250 mm,5 μm),using acetonitrile and water/acetic acid mixture(1 000/1)(gradient elution:0-18 min,26∶74;18-33 min,80∶20) as mobile phase with the rate of 1.0 mL.min-1 at 25 ℃,the wavelength for measurement was 310 nm.Result:The linearity was in the range of 0.1-5.0 μg(n=6),and the correlation coefficient was 0.999 9.Th...