牛磺酸对舒张性心力衰竭大鼠心肌细胞钙超载的拮抗作用

Antagonism for different doses of taurine on calcium overload in myocardial cells of diastole heart failure rat model

目的:观察牛磺酸(Tau)对舒张性心力衰竭(DHF)大鼠心肌细胞内钙离子浓度[Ca2+]i和心肌细胞膜上ATP酶活性的影响。方法:采用腹主动脉缩窄法建立DHF模型,术后4周随机分为模型组,Tau高、中、低剂量组(400,200,100 mg.kg-1.d-1)4组(n=10),另有假手术组10只。连续给药4周,酶解法分离DHF大鼠心肌细胞,以Fluo-3AM荧光指示剂负载,应用激光扫描共聚焦显微镜(LSCM)技术检测心肌细胞内[Ca2+]i变化,[Ca2+]i用荧光强度(FI)表示;通过ATP酶试剂盒,采用酶促反应定磷比色法测定心肌细胞膜上ATP酶活性。结果:与假手术组相比,模型组大鼠心肌细胞内Ca2+浓度明显上升,心肌细胞膜上ATP酶活性明显下降。与模型组比较,Tau高剂量组显著降低心肌细胞内荧光值,明显升高ATP酶活性;Tau中剂量组明显降低心肌细胞内荧光值和ATP酶活性;Tau低剂量组明显升高心肌细胞内荧光值,显著降低ATP酶活性。结论:大剂量的Tau可以明显提高DHF大鼠心肌细胞膜ATP酶活性,改善DHF大鼠心肌细胞内[Ca2+]i,拮抗钙超载。

Objective:To study the effects of different doses of taurine(Tau) on calcium ion concentration([Ca2+]i) and ATPase on cardiocyte membrane of diastole heart failure rats.Method:Diastole heart failure model was established by the coarctation of abdominal aorta.Four weeks after operation,forty diastole heart failure rats were divided into four groups randomly as follows,model(normal saline 2 mL),taurine(400 mg.kg-1.d-1),taurine(200 mg.kg-1.d-1),taurine(100 mg.kg-1.d-1),with 10 rats for each group(n=10),and 10 ...